Cyclic Mixture Mutagenesis \\ for DNA-Based Computing -- New tubes needed<A NAME="2"> </A><A NAME="sec:tubes"> </A>

Go backward to High-level Steps
Go up to Detailed Protocol for Initial Experiment
Go forward to Mixture Recipies

New tubes needed

        tube
qty     size    contents
---     ----    --------
1       50 ml   10X Taq sequencing buffer
1       1.5 ml  500 ul 10 mM EDTA
1       1.5 ml  500 ul 1 mM EDTA
4       1.5 ml  500 ul 1 mM dNTP
4       1.5 ml  500 ul 0.1 mM dNTPs
1       1.5 ml  500 ul 0.01 mM dATP
1       1.5 ml  500 ul dNTP chases
4       1.5 ml  60 ul Taq Sanger Mixes
1       1.5 ml  200 ul 0.1M TrisCL (pH 7.5)/0.1 mM EDTA
3       1.5 ml  \sim50 ul 2.44 uM oligos
3       1.5 ml  20 ul DNA mixes
12      1.5 ml  13.5 ul reaction mixes
36      0.5 ml  4.5 ul reaction mixes

- Michael P. Frank, September 12, 1995. Formatted using HyperLaTeX-1.3.