Starting materials  

oligo O1 (0 mismatches) @ 112 uM in H2O
oligo O2 (2 mismatches) @  91 uM in H2O
oligo O3 (4 mismatches) @  98 uM in H2O
Bluescript KSII, circular double-stranded, \sim1 ug/ul (\sim500 nM) in TE
Taq DNA polymerase (? U/ul)
10 mCi/ml [alpha-S-35]dATP (500 to 1200 Ci/mmol)
100 mM dNTP's
5 mM ddNTP's
1M TrisCl pH 9
1M TrisCl pH 7.5
1M MgCl2
0.5 M EDTA
dH2O
Stop/loading dye