Tools  

  

Barany '91

Francis Barany. "Genetic disease detection and DNA amplification using cloned thermostable ligase." Proc. Natl. Acad. Sci. USA 88:189-193, Jan. 1991.

There is a thermostable ligase that we can use to ligate together new DNA molecules in repeated PCR cycles. It has the interesting property, investigated in this paper, that it will not ligate if the base pairs are mismatched at the neighboring ends. This fact could conceivably be used to force computations to go forward in our "brick-wall" approach.

  

Walker et al. '92

G. Terrance Walker, Michael C. Little, James G. Nadeau and Daryl D. Shank. "Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system." Proc. Natl. Acad. Sci. USA 89:392-396, Jan. 1992.

  

Michael-93

Scott F. Michael. "Mutagenesis by incorporation of a phosphorylated oligo during PCR amplification." BioTechniques, 1993(?).

  

CPMB

Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J. G. Seidman, John A. Smith, and Kevin Struhl, editors. Current Protocols in Molecular Biology. John Wiley & Sons, Inc., 1994.